rat enteric glial cell line Search Results


rat enteric glial cell line  (ATCC)
95
ATCC rat enteric glial cell line
(A) Schematic of GFAP △Men1 -tdTomato construct. (B) Widefield images and (C) quantitation of tdTomato signal in the stomach and duodenum of wild type and GFAP △Men1 mice expressing tdTomato reporter. n=4–5 mice per group; *** p < 0.001, **** p < 0.0001 by unpaired Student’s T-test. (D) Whole tissue mounts of proximal duodenum from Cre negative and tdTomato-expressing mice showing tdTomato fluorescence localized to the myenteric plexus (MP). (E) Representative images of cryosections of corpus (CP), gastric antrum (AT), and proximal duodenum (DUO) from wild type and GFAP △Men1 mice expressing tdTomato. (G) Co-immunoprecipitation (Co-IP) of menin and GFAP from <t>rat</t> EGC whole <t>cell</t> lysate (WCL), and nuclear (N) and cytoplasmic (C) fractions followed by western blot for GFAP and menin, respectively. Input is 5–10% of lysate used for IP. (H) Expression of Men1 and the <t>glial</t> transcripts Gfap and S100b following siRNA-mediated Men1 silencing in cultured rat <t>enteric</t> glial cells. n=3 independent experiments; * p < 0.05, *** p < 0.001, **** p < 0.0001 by unpaired Student’s t-test. Data are represented as mean ± SEM. (I) Western blot of menin and GFAP in whole cell lysates following 72h Men1 silencing in rat enteric glial cells.
Rat Enteric Glial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat enteric glial cell line - by Bioz Stars, 2026-03
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cultures of human and adult rat enteric glial cells (egc)  (Dawley Inc)
90
Dawley Inc cultures of human and adult rat enteric glial cells (egc)
Summary table of the research presented in the discussion.
Cultures Of Human And Adult Rat Enteric Glial Cells (Egc), supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cultures of human and adult rat enteric glial cells (egc)/product/Dawley Inc
Average 90 stars, based on 1 article reviews
cultures of human and adult rat enteric glial cells (egc) - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


(A) Schematic of GFAP △Men1 -tdTomato construct. (B) Widefield images and (C) quantitation of tdTomato signal in the stomach and duodenum of wild type and GFAP △Men1 mice expressing tdTomato reporter. n=4–5 mice per group; *** p < 0.001, **** p < 0.0001 by unpaired Student’s T-test. (D) Whole tissue mounts of proximal duodenum from Cre negative and tdTomato-expressing mice showing tdTomato fluorescence localized to the myenteric plexus (MP). (E) Representative images of cryosections of corpus (CP), gastric antrum (AT), and proximal duodenum (DUO) from wild type and GFAP △Men1 mice expressing tdTomato. (G) Co-immunoprecipitation (Co-IP) of menin and GFAP from rat EGC whole cell lysate (WCL), and nuclear (N) and cytoplasmic (C) fractions followed by western blot for GFAP and menin, respectively. Input is 5–10% of lysate used for IP. (H) Expression of Men1 and the glial transcripts Gfap and S100b following siRNA-mediated Men1 silencing in cultured rat enteric glial cells. n=3 independent experiments; * p < 0.05, *** p < 0.001, **** p < 0.0001 by unpaired Student’s t-test. Data are represented as mean ± SEM. (I) Western blot of menin and GFAP in whole cell lysates following 72h Men1 silencing in rat enteric glial cells.

Journal: bioRxiv

Article Title: GFAP-directed Inactivation of Men1 Exploits Glial Cell Plasticity in Favor of Neuroendocrine Reprogramming

doi: 10.1101/2022.02.10.479845

Figure Lengend Snippet: (A) Schematic of GFAP △Men1 -tdTomato construct. (B) Widefield images and (C) quantitation of tdTomato signal in the stomach and duodenum of wild type and GFAP △Men1 mice expressing tdTomato reporter. n=4–5 mice per group; *** p < 0.001, **** p < 0.0001 by unpaired Student’s T-test. (D) Whole tissue mounts of proximal duodenum from Cre negative and tdTomato-expressing mice showing tdTomato fluorescence localized to the myenteric plexus (MP). (E) Representative images of cryosections of corpus (CP), gastric antrum (AT), and proximal duodenum (DUO) from wild type and GFAP △Men1 mice expressing tdTomato. (G) Co-immunoprecipitation (Co-IP) of menin and GFAP from rat EGC whole cell lysate (WCL), and nuclear (N) and cytoplasmic (C) fractions followed by western blot for GFAP and menin, respectively. Input is 5–10% of lysate used for IP. (H) Expression of Men1 and the glial transcripts Gfap and S100b following siRNA-mediated Men1 silencing in cultured rat enteric glial cells. n=3 independent experiments; * p < 0.05, *** p < 0.001, **** p < 0.0001 by unpaired Student’s t-test. Data are represented as mean ± SEM. (I) Western blot of menin and GFAP in whole cell lysates following 72h Men1 silencing in rat enteric glial cells.

Article Snippet: A rat enteric glial cell line was purchased from ATCC (#CRL-2690) and grown in DMEM supplemented with 10% Fetal Bovine Serum (FBS) and 100U Penicillin-streptomycin (EGC/PK060399egfr, ATCC, Manassas, VA).

Techniques: Construct, Quantitation Assay, Expressing, Fluorescence, Immunoprecipitation, Co-Immunoprecipitation Assay, Western Blot, Cell Culture

(A) Volcano plot of significant DEGs in gastric antra of wild type and GFAP △Men1 mice. (B) Volcano plot of significant DEGs in pooled wild type pituitaries and pitNETs of GFAP △Men1 mice. (C) Heatmaps of significant DEGs mapped to neuroendocrine differentiation in GFAP △Men1 gastric antra and (D) pitNETs. (E) Heatmap of significant DEGs mapped to the neuroglial lineage in GFAP △Men1 gastric antra and (F) pitNETs compared to litter-mate wild type controls. (G) KEGG Ontology Pathway analysis of gastric antra from wild type and GFAP △Men1 mice showing the number of genes mapped to enriched pathways and their level of statistical significance. (H) Immunofluorescent staining for gastrin-releasing peptide (Grp) and synaptophysin (Syp) in WT and GFAP △Men1 gastric antra, shown as green and red respectively. Insets: colocalization of Grp and Syp in the gastric mucosa (yellow). (I) Quantitation of synaptophysin and Grp mRNA following 48h siRNA-mediated silencing of Men1 in rat enteric glial cells. n=3 independent experiments; * p < 0.05 and ** p < 0.01 by unpaired Student’s t-test. Data are represented as mean ± SEM.

Journal: bioRxiv

Article Title: GFAP-directed Inactivation of Men1 Exploits Glial Cell Plasticity in Favor of Neuroendocrine Reprogramming

doi: 10.1101/2022.02.10.479845

Figure Lengend Snippet: (A) Volcano plot of significant DEGs in gastric antra of wild type and GFAP △Men1 mice. (B) Volcano plot of significant DEGs in pooled wild type pituitaries and pitNETs of GFAP △Men1 mice. (C) Heatmaps of significant DEGs mapped to neuroendocrine differentiation in GFAP △Men1 gastric antra and (D) pitNETs. (E) Heatmap of significant DEGs mapped to the neuroglial lineage in GFAP △Men1 gastric antra and (F) pitNETs compared to litter-mate wild type controls. (G) KEGG Ontology Pathway analysis of gastric antra from wild type and GFAP △Men1 mice showing the number of genes mapped to enriched pathways and their level of statistical significance. (H) Immunofluorescent staining for gastrin-releasing peptide (Grp) and synaptophysin (Syp) in WT and GFAP △Men1 gastric antra, shown as green and red respectively. Insets: colocalization of Grp and Syp in the gastric mucosa (yellow). (I) Quantitation of synaptophysin and Grp mRNA following 48h siRNA-mediated silencing of Men1 in rat enteric glial cells. n=3 independent experiments; * p < 0.05 and ** p < 0.01 by unpaired Student’s t-test. Data are represented as mean ± SEM.

Article Snippet: A rat enteric glial cell line was purchased from ATCC (#CRL-2690) and grown in DMEM supplemented with 10% Fetal Bovine Serum (FBS) and 100U Penicillin-streptomycin (EGC/PK060399egfr, ATCC, Manassas, VA).

Techniques: Staining, Quantitation Assay

Summary table of the research presented in the discussion.

Journal: Journal of Clinical Medicine

Article Title: Involvement of Proinflammatory Arachidonic Acid (ARA) Derivatives in Crohn’s Disease (CD) and Ulcerative Colitis (UC)

doi: 10.3390/jcm11071861

Figure Lengend Snippet: Summary table of the research presented in the discussion.

Article Snippet: Pochard et al., 2016 [ ] , CD , Study group ( n = 6) active disease (n=6) men ( n = 3) women ( n = 3) age 19–74 Control group ( n = 6) Sprague Dawley rats , Cultures of human and adult rat enteric glial cells (EGC) , 15-HETE production in EGC CD patients was reduced compared to controls 15-HETE inhibition in rats increased intestinal epithelial barrier permeability.

Techniques: Control, Activity Assay, Concentration Assay, Expressing, Clinical Proteomics, Drug discovery, Inhibition, Permeability